Metronidazole (METRONIDAZOLE) and mupirocinpirocin (MUPIROCIN) both are anti-microbial type of antibiotics. This combination of drugs will be used to treat infections and are applied directly on the wounds. In RP-HPLC method, a mobile phase of phosphate buffer (pH 4): methanol (30:70 v/v) was used to resolve metronidazole and mupirocinpirocin from a mixture. Phenomex C18 column was used and flow rate was selected as 1 ml/min. Detection was carried out at 242 nm which is isosbestic point of metronidazole and mupirocinpirocin. The linearity range obtained for the RP-HPLC method were 5-15 μg/ml and 10 - 30 μg/ml with corresponding correlation coefficient of 0.999 and 0.999 for metronidazole and mupirocinpirocin respectively. First derivative spectrophotometry method that involves estimation of metronidazole at 330.0 nm and mupirocinpirocin at 232.0 nm. In this method linearity evaluated is 2.5 - 12.5 μg/ml for metronidazole and 5 - 25 μg/ml for mupirocinpirocin and correlation coefficients found 0.999 and 0.999 for both the drugs respectively. The method was found to be rapid, accurate and precise. This method was validated according to ICH guidelines.
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